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gsx1xenopus hypothalamus 

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Experiment details for gsx1

Illes JC et al. (2009) Assay

Cloning and expression analysis of the anterior parahox genes, Gsh1 and Gsh2 from Xenopus tropicalis.

Gene Clone Species Stages Anatomy
gsx1 tropicalis NF stage 29 and 30 hypothalamus

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  Figure 1. The Xenopus tropicalis ParaHox cluster. A: Diagram of ParaHox clusters from amphioxus, mouse, human, and X. tropicalis (not to scale). Arrows denote the transcription orientation of the genes. The X. tropicalis cluster diagram indicates exon/intron structure. B: One-colour triple WISH on a stage 30 X. tropicalis embryo with probes for Xt Gsh1, Xt Pdx1, and Xt Cdx2. C: The remnants of the four X. tropicalis ParaHox clusters, showing some of the flanking genes and approximate distances. Gsx genes are shown in green, Xlox in blue, Cdx in red, Chic in pink, and tyrosine kinase receptors in brown.

Gene Clone Species Stages Anatomy
gsx1 tropicalis NF stage 31 hypothalamus
gsx1 tropicalis NF stage 35 and 36 to NF stage 40 hypothalamus
gsx1 tropicalis NF stage 47 hypothalamus

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  Figure 2. Developmental expression of Gsh1 [now called gsx1] . A-J, P-W: Gsh1 WISH. K-O: Double WISH as indicated. L, N, S-V: Cleared embryos. K, M, O-R, S (inset): Microtome sections (14 mu m). A: Dorsal, early neurula stage 14. B: Dorso-anterior, mid-neurula stage 17. C: Dorsal, late neurula stage 19. D: Lateral, stage 19. E: Lateral, early tailbud stage 25. F: Dorsal, stage 25. G: Anterior, stage 25. H-S: Tailbud stage 31. H: Lateral. I: Dorsal. J: Anterior. K: Sagittal section through head. L: Head, lateral. M: Anterior hindbrain, transverse section. N: Head, lateral. O, P: Anterior forebrain, transverse sections. Q: Mid forebrain, transverse section. R: Posterior forebrain, transverse section. S: Head, lateral; inset, anterior forebrain section. T: Head, lateral, tailbud stage 35. U: Anterior spinal cord, lateral, late tailbud stage 40. V: Head, dorsal, larval stage 47. W: Midbrain, transverse section, stage 47. Key: ant, anterior; fmb, forebrain-midbrain boundary; hb, hindbrain; hyp, hypothalamus; lge, lateral ganglionic eminence; mge, medial ganglionic eminence; mhb, midbrain-hindbrain boundary; nc, notochord; ob, olfactory bulb; ov, otic vesicle; post, posterior; pt, pretectum; r1, rhombomere 1; r6, rhombomere 6; se, septum; sc, spinal cord; tec, tectum; tel, telencephalon; th, thalamus; vz, ventricular zone; zli, zona limitans intrathalamica.