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Figure 4. Yap loss of function slows down cell cycle kinetics of retinal stem cells.(A) PH3 immunolabeling on retinal sections from stage 40 tadpoles following two-cell stage microinjection of Yap-5-mismatch-MO (control) or Yap-MO. The CMZ is delineated with dotted lines. (B) Quantification of the mitotic index within the CMZ. The number of analyzed retinas per condition is indicated in each bar. (C) Outline of the PLM and EdU cumulative labeling experiments: tadpoles injected as in (A) were fixed at different time points following EdU injection at stage 39. EdU and PH3 labeling was then analyzed on retinal sections. (D) Retinal sections stained for both PH3 and EdU. The CMZ is delineated with dotted lines. Arrows and arrowheads point to PH3+/EdU+ and PH3+/EdU− cells respectively. (E) Quantification of the PLM within the whole CMZ. TG2: G2-phase duration. (F) Quantification of the EdU labeling index within retinal stem cells along with increasing EdU exposure times. GF: growth fraction; TC: total cell cycle; TS: S-phase. (G) Estimation of TC and TS. Data are represented as mean ± SEM. Scale bar = 40 µm.DOI:
http://dx.doi.org/10.7554/eLife.08488.011 |
