| |
Fig. 3. Effects of Mst1/2 MO injection on embryonic erythrocyte
formation. (A,A�) Erythrocyte marker, _-globin, was normally
expressed in the VBI of embryos injected with standard control
MO (Std. MO, 5 ng). (B-D�) Knockdown of Mst1 (Mst1t MO, 5
ng), Mst2 (Mst2s MO, 5 ng) and Mst1/2 (Mst1t+2s MO, 2.5/2.5
ng) decreased _-globin expression in VBI. (E) At the later stage,
_-globin was detected in circulating erythrocytes in the vein of
standard control MO-injected embryos. (F-H) Knockdown of
Mst1, Mst2 and Mst1/2 inhibited the circulation of erythrocytes.
(I) O-dianisidine staining also visualized circulating erythrocytes in
controls, (K-L) whereas erythrocytes were hardly visualized in Mst1,
Mst2 and Mst1/2 morphants. (M) RT-qPCR analysis revealed that
knockdown of Mst1/2 decreased `-globin expression. Expression
levels were normalized relative to ODC. Values represent means
+ SD of three independent experiments, *P < 0.05, **P < 0.01. |
