|
Fig. 5. Only the border of the neural plate is sensitive to BMP inhibition in Xenopus. (A–I) Smad6 (1 ng) injection into the A4 blastomere induces Pax3 (A–C), Slug (D–F) and Hairy2A (G–I). (A, D, and G: dorsal view; B, C, E, F, H and I: ventral view of the embryo to their left). (J–N) Injection into blastomere A2/3 expands Sox3 (J–L) and Slug (M, N). J, M: dorsal view; K, L and N are lateral views of the embryos to their left. White brackets in J show the extension of the neural plate in the injected and non-injected sides of the embryo. The black square in K indicates the area enlarged in L. Black arrows in L point to injected cells adjacent to the endogenous neural plate, expressing Sox3; the blue arrows point to injected cells distant from the endogenous neural plate, which do not express Sox3. Injected cells were recognized by FDX or LacZ (C, F, I, K, L and N, for embryos to their left). (O–P) Animal caps from Smad6-injected embryos at the 2-cell stage express Sox3 (O), which is not inhibited by CerS (P). |