| |
Figure 1. Pattern of hairy2 in Tailbud Stage Embryos(A) Whole-mount in situ hybridization for hairy2a RNA in a cleared stage 28 embryo. Expressing tissues include: PSM Stripe (the anterior presomitic mesoderm stripe), FP (neural tube floorplate), RP (neural tube roofplate), Br.A (branchial arches), PN (pronephros), FM (fin mesenchyme), as well as the brain and eye.(B) Similar in situ in an uncleared embryo.(C) In situ for hairy1.(D) In situ for hairy2a in a stage 26 embryo (plastic embedded and imaged with DIC optics). Arrowheads indicate somite borders.(E–H) Proposed order of hairy2a PSM stripe patterns (dorsal is top, anterior is left).(I) hairy2a PSM stripes in three adjacent presumptive somites (magenta, marked by arrows).(J) (left) and (K) (right) sides of the same embryo showing different patterns for hairy2a (the left side has been flipped to be in the same orientation as the right side). A similar pair of images from another embryo is shown in (L) and (M) |
