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FIG. 1. Comparison of X-Notch-1 and E(spl)-related gene expression patterns in stage 40 Xenopus retina. (A) Comparison of X-Notch-1 and ESR1 expression patterns. Double in situ hybridizations were performed on sections of stage 40 Xenopus retina using DIG-labeled ESR1 probe and fluorescein-labeled X-Notch-1 probe. ESR1 expression was viewed under visible light (deep purple) and X-Notch-1 expression was viewed under fluorescence (red). A and B show the same section. (A) ESR1 expression is detected in the CMZ except in cells in the most peripheral region. (B) X-Notch-1 labeling is also detected in the CMZ except in cells in the most peripheral region. Occasional labeled cells are seen in the central retina, in late born cells (Dorsky et al., 1995). C and D show a higher magnification of the CMZ from the sections depicted in A and B, respectively. (E) Double labeling demonstrates that ESR1 and X-Notch-1 expressions overlap in the CMZ. CMZ, ciliary marginal zone; CR, central retina; L, lens. Scale bar in A and B, 100 um; scale bar in C, D, and E, 30 um. (F) Comparison of X-Notch-1 and ESR3 expression patterns. Double in situ hybridizations were performed as above with ESR3 in deep purple and X-Notch-1 in red. (F) ESR3 expression is detected in the CMZ except in cells in the most peripheral region. (G) X-Notch-1 labeling is also detected in the CMZ except in cells in the most peripheral region. H and I show a higher magnification of the CMZ from the sections depicted in F and G, respectively. (J) Double labeling demonstrates that ESR3 and X-Notch-1 expressions overlap in the CMZ. Scale bar in F and G, 100 um; scale bar in H, I, and J, 30 um |
