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Figure 3. Posterior Hox gene expression in regenerating limbs. Stage 54 limbs were amputated at knee level and left to regenerate for 1 (A,E,I), 3 (B,F,J), or 5 (C,G,K) days before they were subjected to in situ hybridisation. Nonregenerating stage 57 limbs were amputated at knee level and left to heal for 5 days (H,L). D: Stage 54 limb was wounded in the flank and left to heal for 1 day (black arrow points to the wound). A-D: XHoxc10 expression, (E-H) XHoxa13 expression, and (I-L) XHoxd13 expression. Hash marks indicate level of resection. Looking down dorsally, proximal to the left and anterior down. |
