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Figure 5. Expression of HSP60 in regenerating WT and N1 limbs and during development Expression of HSP60 in regenerating WT and N1 limbs and during development. Gene expression in regenerating WT and N1 limbs and embryo tissue. (A-J) In situ hybridisation showing Hsp60 expression in the regeneration bud. (M-P) Unoperated limb buds illustrating Hsp60 expression during limb development. (K) In situ hybridisation showing Hsp60 expression in stage 57 hindlimb of a WT animal 2 days after amputation. (L) In situ hybridisation showing Hsp60 expression in stage 32 embryo. White arrowheads indicate approximate amputation plane, scale bar in A applies to panels A-J and scale bar in P applies to panels M-P. In limb pictures (A-K, M-P) posterior is uppermost, and distal to the left, dr = days of regeneration. In L, anterior is to the left and dorsal uppermost. |
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hspd1 (heat shock 60kDa protein 1 (chaperonin)) gene expression in Xenopus laevis embryo, via in situ hybridization, NF stage 32 embryo, lateral view, anterior left, dorsal up. |
