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Fig. 2. Whole-mount in situ hybridization. Expression of Xhyal2 during Xenopus embryogenesis. (A) Mid-sagittal section of a gastrula stage embryo, animal side up, dorsal side to the right; bc, blastocoel; bp, dorsal blastopore. (B) Neurula stage 17 embryo, dorsolateral view, anterior side to the right; hf, head folds. (C) Longitudinal section of neurula stage 17 embryo, anterior side up; a, archenteron; e, epidermis; n, notochord; nf, neural fold; pm, prechordal mesoderm. (D) Tailbud stage 28 embryo, lateral view, anterior side to the left; cg, cement gland; pn, pronephros. (E) Tadpole stage 37, lateral view; pcv, posterior cardinal vein. (F) Close-up view of the heart and ear region of a stage 30 embryo rendered transparent by mounting in tetrahydro-naphthaline; ea, eye anlagen; ov, otic vesicle; black triangles mark precursors of the common cardinal veins. (G,H) Close-up views of embryo shown in (E). (G) Nephric region; white triangles mark the forming ureteric duct originating from the pronephros (pn); black triangles mark the intersomitic vessels that end in the posterior cardinal vein; pv, prevascular endothelial cell. (H) Specimen rendered transparent by mounting in tetrahydro-naphthaline; trunk region; black triangles mark the medial part of the notochord. |
