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Fig. 3. Identification of Id gene expressing cells in the blastema. The expression of the Id family was examined by in situ
hybridization. (A,B) Id2 expression in the late bud stage of the
newt blastema. (C,D) Id3 expression in the late bud stage of the
newt blastema. (E,F) Id2 expression in early-medium bud stage of
the Xenopus blastema. (G,H) Id3 expression in early-medium bud
stage of the Xenopus blastema. Anti-sense probes were used for
the detection of transcripts in (A,C,E,G) and sense probes in
(B,D,F,H). Large closed arrowheads in (A,E) indicate the Id2-
expressing epidermal cells. Open arrowheads in (A) indicate the Id-
2-expressing mesenchymal cells near the wound epidermis. Small
closed arrowheads in (C,G) indicate the Id3-expressing cells in
precartilage condensations. Bar, 200 μm. |
