|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
me2
|
|
tropicalis
|
NF stage 49
to
NF stage 55
|
spinal cord
,
muscle
,
dorsal tail fin
,
blood vessel
,
tail somite
,
[+]
|
nadk
|
|
tropicalis
|
NF stage 49
to
NF stage 55
|
notochord
,
muscle
,
dorsal tail fin
,
blood vessel
,
tail somite
,
[+]
|
g6pd
|
|
tropicalis
|
NF stage 49
to
NF stage 55
|
spinal cord
,
muscle
,
dorsal tail fin
,
blood vessel
,
tail somite
,
[+]
|
me3
|
|
tropicalis
|
NF stage 49
to
NF stage 55
|
notochord
,
muscle
,
dorsal tail fin
,
blood vessel
,
tail somite
,
[+]
|
idh2
|
|
tropicalis
|
NF stage 49
to
NF stage 55
|
muscle
,
dorsal tail fin
,
blood vessel
,
ventral tail fin
,
regenerating tail
|
|
|
Figure 5. Systematic analysis of intracellular metabolic processes using gene ontology. (A) 66 metabolic processes are upregulated in the T24h array (gray). Five of ten highest upregulated processes in the T24h array are colored. The key to this graphic is shown below and "N" indicates the number of genes in the array corresponding to each process. "Organic acid metabolism", which was identified in an initial GO analysis (Figure 4D) is also plotted on the graph. (B) Nicotinamide, NADP, and the generation of reactive oxygen species (ROS) are connected by the metabolic pathway shown in (B) (genes regulating the pathway shown in red, proteins in green). (C) Shows the in situ hybridization patterns of g6pd, idh1, idh2, me2, me3, and nadk following tail regeneration (0 h, 1 h, 6 h, 24 h, and 60 h). (D) RT-qPCR expression profile of nadk (blue) and g6pd (red) following tail amputation. Error bars represent standard error of mean (SEM), lines connecting each error bar are meant to serve as a visual aide. |