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Figure 3. Downregulation of non-transcription factor candidate target genes after knockdown of EBF2 and EBF3. One cell of two-cell stage embryos was injected with either control MO, both EBF2 MO and EBF3 MO (2MO + 3MO), or dominant negative Xenopus EBF3 (DN-XEBF) mRNA. β-Galactosidase (β-gal) mRNA was co-injected as a marker of the injected side. In all panels the right side is the injected side, showing the light blue color of X-gal staining. (A-R) The expression of pcdh8 (B,C), peripherin (E,F), greb1 (H,I), nf-m (K,L), kcnk5 (N,O), and activin beta b (Q,R) is downregulated by EBF2 MO and EBF3 MO, and by DN-XEBF (brackets), while control MO does not change their expression levels (A,D,G,J,M,P). Panels (A-I) and (P-R) are neurula stage embryos (stage 15/16), and (J-O) are tail bud stage embryos (stages 25 to 28). All panels show dorsal views. |
