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Figure 1. xKazrin expression during Xenopus development. A–C: xKazrin spatio-temporal expression was assessed by means of in situ hybridization of late gastrula (A), neurula (B), and tadpole stage embryos (C). The probe consisted of Dig-labeled anti-sense RNA of full-length xKazrinA, while the negative control was the sense counterpart. D: Kazrin mRNA expression in the head region of tadpoles shown in (C) were visualized using microtome sectioning. Brain neural tube (NEU) and eye (Eye) are indicated. E: Extensive Kazrin mRNA signal was obtained upon prolonged substrate reaction. Right panel shows enlarged image of the head region. Pharyngeal arch (PA) is indicated by an arrowhead. F:Xenopus embryo lysates taken from varying stages as indicated (0.5 embryo/ lane) were subject to SDS-PAGE and immuno-blotted for xKazrin (polyclonal antibody directed against carboxyl 147 amino acids of xKazrinA and IgG purified), or for GAPDH (glyceraldehyde-3-phosphate dehydrogenase; loading control (bottom panel). G: Immuno-blot of Xenopus adult tissues (SDS-soluble fraction) as indicated. xKazrin was detected as 47 kDa (KazrinA or B) and 40 kDa (KazrinC/D) isoforms. Equal total protein was loaded per lane, and actin used as a loading control. Molecular weights are indicated in kilodaltons (kDa).Download figure to PowerPoint |
