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Figure 2. WISH for KCNJ2 in Xenopus and mouse embryos
Species are indicated on the left. A, a dorsal-anterior view of a stage 14 Xenopus embryo dehydrated in methanol following fixation. The KNCJ2 signal is much lighter in the midline and two oval areas on either side at the anterior end, the area outlined by white dots. B, a neurulating Xenopus embryo showing KCNJ2 signal on the anterior neural folds (lower green arrows). Upper green arrow points to expression at the approximate position of the second pharyngeal cleft. Inset is sense strand control. C, stage 27 Xenopus embryo showing strong KCNJ2 staining in the somites (green arrows). Inset is sense strand control. D, in situ hybridization of KCNJ2 mRNA expression in an E8.5 mouse embryo (dorsal view of whole-mount embryo). KCNJ2 mRNA is detected in the midline of the fusing neural tube, and in a punctate bilateral expression pattern lateral to the midline and in anterior neural tissues (green arrows). E, lateral view of whole-mount embryo at E9.5 showing KCNJ2 mRNA expression in the craniofacial region including the frontonasal prominence (fnp) and branchial arches 1 and 2, and in the forming somites (green arrows). F, sectioned ISH of E9.5 mouse embryo revealed KCNJ2 expression in the epithelium of the frontonasal prominence (fnp) (green arrows). A schematic in the right top corner indicates the approximate plane of section. G, lateral view of whole-mount embryo craniofacial region at E10.5 revealed KCNJ2 expression in the frontonasal prominence (fnp), in the mandibular process (ma) (green arrow) including along the border with the maxillary process (mx). H and I, sectioned ISH revealed KCNJ2 mRNA expression in neural crest-derived mesenchymal cells of the palatal shelves (ps), in mesenchymal cells surrounding the developing Meckel's cartilage (m) and in the olfactory epithelium of nasal cavity (nc) in E13.5 (H) and E15.5 (I) embryos (green arrows). Inserts adapted from d'Amaro et al. (2012). J, at E12.5 KCNJ2 mRNA was detected in condensations of the developing forelimb digits (green arrows). Abbreviations: ba1, branchial arch 1; ba2, branchial arch 2; fb, forebrain; fnp, frontonasal prominence; m, Meckel's cartilage; mb, midbrain; ma, mandibular process; mx, maxillary process; nc, nasal cavity; ns, nasal septum; ps, palatal shelf; t, tongue. |
