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Gene/Clone	Species	Stage	Anatomy Item	Experimenter
kctd15	xenopus		ectoderm [+] Bowman's gland,Eberth-Kastschenko layer,Leydig cell,Mauthner cell,Rathke's pouch,Rohon-Beard neuron,accessory olfactory bulb,adenohypophyseal placode,adenohypophysis,afferent neuron,amphibian papilla,amygdala,anterior amygdala,anterior branchial crest,anterior commissure,anterior crista,anterior entopeduncular nucleus,anterior entopeduncular region,anterior hypothalamus,anterior neural fold,anterior neural ridge,anterior neural tube,anterior neuroectoderm,anterior placodal area,anterior tectum,anterior thalamic nucleus,anterodorsal tegmental nucleus,anteroventral tegmental nucleus,astrocyte,astrocyte end-foot,auditory hair cell,auditory ossicle,auditory tube,axon,axon microtubule bundle,basal forebrain,basal ganglion,basilar papilla,bed nucleus of the stria terminalis,brain ventricle,branchial crest,buccopharyngeal membrane,cardiac neural crest,caudal tuberal region,cell body layer of the optic tectum,central amygdala,central nucleus of the thalamus,cerebellum,chordal neural crest,chordal neural plate,chordal neural plate border,chordoneural hinge,cloaca,cloacal sphincter,cranial neural crest,cranial placode,dendrite,dermis,diencephalon,dorsal column nucleus,dorsal fin margin,dorsal fin vasculature,dorsal habenular nucleus,dorsal pallidum,dorsal pallium,dorsal septum,dorsal tegmentum,dorsal telencephalon,dorsal thalamus,dorsolateral neural tube,efferent neuron,eminentia thalami,endolymphatic duct,enteric neuron,epidermal cell,epidermis,epidermis inner layer,epidermis outer layer,external plexiform layer of the olfactory bulb,fin crest,fin margin,fin vasculature,footplate of the stapes,forebrain,forebrain-midbrain boundary,fourth ventricle,frontal nerve,glomerular layer of the olfactory bulb,goblet cell,granule cell,granule cell layer of the cerebellum,granule cell layer of the olfactory bulb,growth cone,habenula,hatching gland cell,hatching gland primordium,hindbrain,hyoid crest,hypophysis,hypothalamus,infundibulum,inner ear,inner fin,interneuron,ionocyte,isthmic nucleus,keratinocyte,lagena,lateral amygdala,lateral crista,lateral forebrain bundle,lateral ganglionic eminence,lateral hypothalamic nucleus,lateral pallium,lateral preplacodal ectoderm,lateral septum,laterodorsal tegmental nucleus,lens epithelium,lens fiber cell mass,lens placode,lens transitional zone,locus coeruleus,magnocellular preoptic nucleus,mammilary region,mandibular crest,mandibular nerve,maxillary nerve,medial amygdala,medial ganglionic eminence,medial pallium,medial septum,medulla oblongata,melanotrope,mesencephalic artery,mesencephalic vein,metencephalic artery,metencephalic vein,midbrain,midbrain tegmentum,midbrain-hindbrain boundary,middle ear,migrating macrophage,migratory neural crest cell,migratory trunk neural crest cell,mitral cell,mitral cell layer of the olfactory bulb,mucociliary epithelium,nasociliary nerve,neural crest,neural fold,neural groove,neural plate,neural plate border,neural stem cell,neurenteric canal,neurocoel,neuroectoderm inner layer,neuroectoderm outer layer,neurohypophysis,neuromast mantle cell,neuromast support cell,neuron projection,neuropil of the optic tectum,notoplate,nucleus accumbens,nucleus of the diagonal band,nucleus of the solitary tract,nucleus reticularis inferior,nucleus reticularis medialis,nucleus reticularis superior,oculomotor motornucleus,olfactory bulb,olfactory sensory neuron,oligodendrocyte,optic chiasm,optic field,optic stalk,optic tectum,optic tract,oral cavity,oral epithelium,osseous labyrinth,otic capsule,outer fin,oval window,pallidum,pallium,pars distalis,pars externa of the stapes,pars intermedia,pars interna of the stapes,pars media of the stapes,pars nervosa,peripheral nerve,pia mater,pial cell,pigment cell,pineal gland,posterior branchial crest,posterior commissure,posterior crista,posterior hypothalamus,posterior neural fold,posterior neural tube,posterior placodal area,posterior tectum,posterior wall of neurenteric canal,posterodorsal tegmental nucleus,posteroventral tegmental nucleus,postmigratory neural crest cell,pre-chordal neural plate,pre-chordal neural plate border,premigratory neural crest cell,preoptic area,preplacodal ectoderm,pretectum,prethalamus,prethalamus basal plate,prootic bone,prosomere,prosomere 4,prosomere 5,prosomere 6,radial glial cell,raphe nucleus,rectal diverticulum,retromammillary area,rhombic lip,rhombomere,roof of mouth,rostral tuberal region,saccular macula,sacral neural crest,semicircular canal,sensorial layer of neurectoderm,skin gland,small secretory cell,solitary tract,stapes,stratum compactum,stratum spongiosum,stria terminalis,striatum,subcommissural organ,subpallium,subthalamic nucleus,sulcus limitans of neural tube,superficial layer of neurectoderm,suprachiasmatic nucleus,supraoptoparaventricular region,telencephalon,telencephalon septum,thalamus,third ventricle,torus semicircularis,trochlear motornucleus,trunk neural crest,tuberal region,tympanic annulus,tympanic disk,vagal neural crest,ventral fin vasculature,ventral habenular nucleus,ventral hypothalamic nucleus,ventral pallidum,ventral pallium,ventral septum,ventral tegmentum,ventricular layer of the optic tectum,ventricular zone,ventrolateral neural tube,ventrolateral thalamic nucleus,ventromedial thalamic nucleus,vestibular apparatus,vestibule,zona limitans intrathalamica,Jacobson's organ,Rathke's pouch,adrenal medulla,afferent neuron,anterior branchial crest,anterior neural ridge,anterior neuroectoderm,astrocyte,astrocyte end-foot,auditory apparatus,brain,branchial crest,buccopharyngeal membrane,caudal lateral line,cement gland,cement gland primordium,choana,chordal neural crest,circumpharyngeal ridge,claw,claw primordium,cloacal sphincter,cornea,cranial placode,dorsal lateral line,dorsal tail fin,dorsolateral placode region,ectodermal cell,efferent neuron,enteric nervous system,enteric neuron,epibranchial placode,epidermis,fin,fin margin,gasserian ganglion,glial cell,hatching gland,hatching gland primordium,hypobranchial placode,hypophyseal primordium,hypophysis,lateral preplacodal ectoderm,lens,lens placode,melanoblast,melanophore,middle lateral line,migrating neuroblast,migratory neural crest cell,mouth,mouth primordium,neural crest,neural fold,neural groove,neural plate border,neural stem cell,neural tube,neuroectoderm,neurogenic placode,neuron,non-ciliated epithelial cell,non-neural ectoderm,non-neurogenic ectodermal placode,oculomotor motornucleus,olfactory epithelium,olfactory nerve,olfactory organ,olfactory pit,olfactory placode,oligodendrocyte,otic capsule,otic placode,otic vesicle,peripheral ganglion,pigment cell,posterior branchial crest,preplacodal ectoderm,proctodeum,radial glial cell,regenerating lens,sacral neural crest,secretory epithelial cell,sensorial layer,skin,small secretory cell,stomodeal-hypophyseal primordium,tail bud,tooth,trigeminal ganglion,trigeminal nerve,trigeminal placode,trunk neural crest,trunk placode,unicellular gland,vagal neural crest,vagus nerve,ventral lateral line,vestibulocochlear ganglion,zona limitans intrathalamica

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Experiment details for kctd15

Takahashi C et al. (2012) Assay

Identification and characterization of Xenopus kctd15, an ectodermal gene repressed by the FGF pathway.

Gene	Clone	Species	Stages	Anatomy
kctd15.L	image:5440283	laevis	NF stage 10.5	ectoderm

kctd15 (potassium channel tetramerization domain containing 15) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 10.5, horizontal view, animal up.

Gene	Clone	Species	Stages	Anatomy
kctd15.L	image:5440283	laevis	NF stage 14	non-neural ectoderm , neural crest
kctd15.L	image:5440283	laevis	NF stage 16	cranial neural crest , anterior neural ridge , neural crest , preplacodal ectoderm
kctd15.L	image:5440283	laevis	NF stage 17	cranial neural crest , neural crest
kctd15.L	image:5440283	laevis	NF stage 18	cranial neural crest , olfactory placode , neural crest
kctd15.L	image:5440283	laevis	NF stage 25	olfactory placode , trigeminal ganglion , anterior lateral line placode

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Fig. 2 Spatial expression of Xenopus kctd15 in early development. Whole mount in situ hybridization against kctd15 was performed on embryos from indicated stages. No detectable signal was seen with the sense probe (data not shown). (A-C) At stage 10.5, kctd15 was uniformly expressed throughout the presumptive ectoderm. (D-G) At stage 14, kctd15 expression was detected in the non-neural ectoderm and was excluded from the neural plate. Dorsal is upward in D and E. Anterior is upward in F and G. (H-J) At stage 16, kctd15 expression was detected in the preplacodal ectoderm (ppe), the neural crest (nc) and the roof plate (rf). Dorsal is upward in H and I. Anterior is upward in J. (K-M) At stage 18, kctd15 expression was detected in the neural crest (nc), the roof plate (rf) and the olfactory placode (pOl). Dorsal is upward in K and L. Anterior is upward in M. (N-P) At stage 25, kctd15 expression was detected in the pronephric duct (pd), the mandibular arch (Md), the hyoid arch (Hy), the olfactory placode (pOl), trigeminal ganglia (tg) and the anterodorsal lateral line placode (pAD). Anterior is left. (Q) Whole mount double in situ hybridization against kctd15 and sox10 was performed on stage 17 embryos (middle and right panels). The right panel is an enlarged view of the middle panel. Dark blue and orange dotted lines mark the kctd15-expressing and sox10-expressing area, respectively. For reference, single in situ hybridization against sox10 (red purple) was also performed (the left panel). All panels are dorsal views with anterior to the top.

Gene	Clone	Species	Stages	Anatomy
kctd15.L	image:5440283	laevis	NF stage 15	cranial neural crest , neural crest

Fig. 3. FGF signaling inhibits Xenopus kctd15 expression in vivo. (A) FGF represses kctd15 expression indirectly. Animal caps were dissected at stage 10.25, treated with FGF (100 ng/ml) for 3 hours in the presence or absence of cycloheximide (CHX; 5 mg/ml), and then harvested for real-time quantitative RT-PCR analysis. Shown is the average of two independent experiments. The error bar represents SD. Significant differences are marked by asterisks (*p < 0.05, unpaired t test). P values (FGF treatment versus no FGF treatment) were as follows: 0.024 for kctd15 expression in the absence of CHX, 0.80 for kctd15 expression in the presence of CHX, 0.23 for xbra expression in the absence of CHX, and 0.24 for xbra expression in the presence of CHX. (B) Inhibition of FGF signaling increases the abundance of kctd15 mRNA. XFD mRNA (1 ng) was injected into animal poles at 2-cell stage, and injected embryos were harvested at stage 11 for real-time quantitative RT-PCR analysis (left). Embryos were treated with indicated doses of SU5402 at stage 9, and then harvested at stage 11 for real-time quantitative RT-PCR analysis (right). Shown is the average of two (left) or three (right) independent experiments. The error bar represents SD. *P < 0.05, unpaired t test. P values versus control were as follows: 0.14 for XFD mRNA-injected embryos, 0.031 for SU5402 (50 mM)-treated embryos, and 0.015 for SU5402 (100 mM)-treated embryos. (C) Inhibition of FGF signaling dramatically expands the region expressing kctd15. Embryos were treated with indicated doses of SU5402 at stage 9, fixed at stage 15, and then subjected for whole mount in situ hybridization against kctd15. Upper panels show dorsal views with anterior toward the top. Lower panels show lateral views with anterior to the left. White arrowheads indicate unclosed blastopores, which are commonly observed phenotypes caused by inhibition of the FGF pathway (Amaya et al., 1991; Chung et al., 2004).

Gene	Clone	Species	Stages	Anatomy
kctd15.L	image:5440283	laevis	NF stage 16	cranial neural crest , neural crest

kctd15 (potassium channel tetramerization domain containing 15) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 16, lateral view, anterior left, dorsal up.

Gene	Clone	Species	Stages	Anatomy
kctd15.L	image:5440283	laevis	NF stage 25	olfactory placode , mandibular crest , trigeminal placode , trigeminal ganglion , anterodorsal lateral line placode

kctd15 (potassium channel tetramerization domain containing 15) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 25, lateral view, anterior left, dorsal up.