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Display additional annotations [+]
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Figure 2 Classification of specific retinal markers retrieved from the screen. Schematic diagram illustrates the chosen subdivisions of the tadpole retina. n indicates the number of markers retrieved from the screen that exhibit specific expression in only one of these compartments. Pictures below are stage 39 transverse retinal sections (dorsal up) that provide typical examples of such markers, with a correspondence to the schematic lettering (A). A fuzzy staining in the CMZ that invades the nuclear layers (A0) is not considered CMZ-specific. White dotted lines delineate the staining. Gene symbol is indicated under each panel. Scale bar = 50 um. Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.
This material is reproduced with permission of John Wiley & Sons, Inc. |
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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
spry4
|
|
tropicalis
|
NF stage 39
|
retina
,
hindbrain
,
midbrain-hindbrain boundary
,
ciliary marginal zone
,
pharyngeal arch
,
[+]
|
hes4
|
|
tropicalis
|
NF stage 39
|
retina
,
forebrain
,
midbrain
,
hindbrain
,
midbrain-hindbrain boundary
,
[+]
|
dnajc27
|
|
tropicalis
|
NF stage 39
|
retina
,
midbrain
,
hindbrain
,
midbrain-hindbrain boundary
,
stomodeal-hypophyseal primordium
,
[+]
|
ranbp1
|
|
tropicalis
|
NF stage 39
|
retina
,
hindbrain
,
midbrain-hindbrain boundary
,
ciliary marginal zone
,
pharyngeal arch
,
[+]
|
aurka
|
|
tropicalis
|
NF stage 39
|
retina
,
midbrain
,
midbrain-hindbrain boundary
,
ciliary marginal zone
,
pharyngeal arch
|
fndc3b
|
|
tropicalis
|
NF stage 39
|
otic vesicle
,
retina
,
midbrain-hindbrain boundary
,
ciliary marginal zone
,
pharyngeal arch
,
[+]
|
sulf2
|
|
tropicalis
|
NF stage 39
|
retina
,
forebrain
,
midbrain
,
hindbrain
,
midbrain-hindbrain boundary
,
[+]
|
mgrn1
|
|
tropicalis
|
NF stage 39
|
otic vesicle
,
retina
,
midbrain-hindbrain boundary
,
ventral blood island
,
ciliary marginal zone
,
[+]
|
zmcm3
|
|
tropicalis
|
NF stage 39
|
otic vesicle
,
hindbrain
,
midbrain-hindbrain boundary
,
pronephric duct
,
ciliary marginal zone
,
[+]
|
ssbp1
|
|
tropicalis
|
NF stage 39
|
otic vesicle
,
retina
,
midbrain
,
midbrain-hindbrain boundary
,
pronephric duct
,
[+]
|
trmt2a
|
|
tropicalis
|
NF stage 39
|
retina
,
midbrain-hindbrain boundary
,
ciliary marginal zone
,
pharyngeal arch
,
foregut
,
[+]
|
nop58
|
|
tropicalis
|
NF stage 39
|
retina
,
midbrain
,
midbrain-hindbrain boundary
,
ciliary marginal zone
,
pharyngeal arch
,
[+]
|
alpl
|
|
tropicalis
|
NF stage 39
|
head region
,
eye
,
lateral plate mesoderm
|
ncapg
|
|
tropicalis
|
NF stage 39
|
retina
,
hindbrain
,
midbrain-hindbrain boundary
,
ciliary marginal zone
,
pharyngeal arch
,
[+]
|
gxylt1
|
|
tropicalis
|
NF stage 39
|
otic vesicle
,
retina
,
hindbrain
,
midbrain-hindbrain boundary
,
ciliary marginal zone
,
[+]
|
mhc1-uda
|
|
tropicalis
|
NF stage 39
|
retina
,
hindbrain
,
ciliary marginal zone
,
pharyngeal arch
,
foregut
,
[+]
|
mcmbp
|
|
tropicalis
|
NF stage 39
|
forebrain
,
midbrain
,
midbrain-hindbrain boundary
,
ciliary marginal zone
,
pharyngeal arch
,
[+]
|
|
|
Figure 4 Expression of novel retinal stem cell markers in whole tadpoles. Whole mount expression patterns on stage 39 tadpoles of the 18 retinal stem cell markers. Most clones exhibit a strikingly similar expression profile, strongly suggesting that they form a synexpression group. In particular, in addition to the eye, selected clones label the midbrainindbrain boundary (arrows). The tip of the tail is also stained in most of these pictures. Gene symbol is indicated on each panel. Scale bar = 500um. Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.
This material is reproduced with permission of John Wiley & Sons, Inc. |
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|
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kpnb1 (karyopherin (importin) beta 1) gene expression in Xenopus tropicalis embryos, NF stage 39, as assayed by in situ hybridization, lateral view, anterior left, dorsal up. |