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Fig. 2. Temporal and spatial expression patterns of Xatv. (a) RT-PCR analysis was performed at various developmental stages. Lanes indicate stages according to Nieuwkoop and Faber (1956). The Xatv transcripts first appeared at the late blastula stage (stage 9), peaked during the gastrula stage (stage 10,11,12) and were detected through the neurula to the tadpole stage (stage 13–35). (b–i) Whole-mount in situ hybridization revealed the spatial expression patterns of Xatv. (b) Dorsal view of stage-9 embryo. Xatv transcripts are present in the dorsal marginal zone. (c) Vegetal view of stage-9.5 embryo; transcripts are detected around the marginal zone. (d) Vegetal view of stage-10.5 embryo; the dorsal lip was indicated by an arrowhead. (e) Dorsal view of stage-11 embryo; during gastrulation, the transcripts become gradually restricted to the dorsal midline. (f) Dorsal view of stage-12 embryo; the transcripts were detected only in the dorsal midline. (g) Posterior view of stage-20 embryo; the expression was shifted posteriorly. (h) Dorsal view of stage-25 embryo. asymmetric expression was seen in left LPM. (i) Ventral view of stage-28 embryo; the expression was shifted ventrally. |
