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Fig. 2.
MGPY stains strongly the proliferating adult intestinal stem cells.
Premetamorphic stage 54 tadpoles treated with 10 nM T3 for 0, or 6 days were sacrificed one hour after injecting EdU. Cross-sections of the intestine from the resulting tadpoles were double-stained for EdU (5-ethynyl-2′-deoxyuridine) and with MGPY (methyl green pyronin Y, a mixture of methyl green, which stains DNA, and pyronin Y, which stains RNA [42], [145] and [146]) (A) or for Edu and Lgr5 (in situ hybridization) (B). The approximate epithelium-mesenchyme boundary was drawn based on morphological differences between epithelial cells and mesenchyme cells in the pictures of the double-stained tissues (dotted lines). Note that the clusters (islets) of EdU labeled cells in the epithelium after 6 days of T3 treatment were strongly stained by MGPY and had high levels of Lgr5 mRNA, a well-established marker for adult intestinal stem cells in vertebrates. See [40] for more detail. |
