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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
actc1
|
|
laevis
|
NF stage 29 and 30
|
presomitic mesoderm
,
somite
,
heart
,
muscle
,
epaxial muscle
|
tmod2
|
|
tropicalis
|
NF stage 29 and 30
|
|
tmod3
|
|
tropicalis
|
NF stage 29 and 30
|
|
tmod1
|
|
laevis
|
NF stage 29 and 30
|
somite
,
heart
,
muscle
,
epaxial muscle
|
lmod1
|
|
laevis
|
NF stage 29 and 30
|
somite
,
heart
|
lmod3
|
|
laevis
|
NF stage 29 and 30
|
somite
,
muscle
,
skeletal muscle
,
epaxial muscle
|
tmod4
|
|
tropicalis
|
NF stage 29 and 30
|
somite
,
muscle
,
skeletal muscle
,
epaxial muscle
|
|
|
Fig. 1. Structure and developmental expression of the
leiomodin (Lmod) and tropomodulin (Tmod) gene families.
(A) leiomodin (Lmod1–3) and tropomodulin (Tmod1–4) proteins are
structurally related, yet possess unique domains. Lmods and Tmods
share actin-binding (A1, A2), tropomyosin-binding (TM1) and
leucine-rich-repeat (LRR) domains. A second tropomyosin-binding
domain (TM2) is unique to the Tmods. Lmod proteins have a Cterminal
extension that contains a third actin binding/WH2 domain
(WH2/A3). (B–I) Lmod (C–E) and Tmod (F–I) family gene expression
was analyzed during Xenopus embryonic development using in situ
hybridization. All embryos are positioned with heads to the left.
Expression patterns are shown at the early tailbud stage (st30),
during the early stages of myofibrillogenesis. Earlier expression
during the neurula stage (st15) is presented in supplementary
material Fig. S1. Expression of the definitive striated muscle marker
cardiac actin, actna1 (B), was used to indicate cardiac and skeletal
muscle tissue in the embryo. Arrows indicate developing skeletal
muscle and heart tissues. Only lmod3 (E) and tmod4 (I) are
expressed at high levels in developing skeletal muscle. |
|
|
|
lmod2 (leiomodin 2 (cardiac)) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 34, lateral view, anterior left, dorsal up. |