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FIG. 4. Lmx-1 expression in regenerating hindlimb buds (A) of tadpoles and forelimbs of froglets (indicated by arrowheads). Lmx-1 expression in regenerating blastema is detected at 3 (C), 5 (E), and 7 days (G) after amputation at the presumptive ankle level of stage 52 limb buds, but in stage 55 blastemas (B, D, F, and H) Lmx-1 is detectable only at 7 days after amputation (H). Lmx-1 expression in the blastema after amputation of a froglet forelimb at wrist level (I and J) at 7 (I) and 14 days (J). Lines show the predicted amputation level. Bar, 150 ﰁm. |