|
Fig. 8. Xpygo-2 / MOs inhibit brain development. Immunostaining of tadpoles (st. 43) coinjected with Xpygo-2 MO and Xpygo-2 MO (20 ng each)
using the pan-neural antibody 2G9 (A, dorsal view; B, lateral view) indicated anterior neural truncations with loss of retinal tissue as well as fore-, mid-, and
hindbrain defects (arrows). These structures were rescued by coinjection of Xpygo-2 / RNA (1 ng each; D, dorsal view; E, lateral view). Xpygo-2 MO
alone (40 ng) generated severe neural deficits rostral to and including the hindbrain (F, dorsal view; G, lateral view; arrows), while Xpygo-2 MO alone (40
ng) primarily disrupted and reduced neural tissue rostral to and including the midbrain (I, dorsal view; J, lateral view; arrows). Control MO (40 ng)-injected
embryos showed normal 2G9 immunostaining of the eyes (e), forebrain (f), midbrain (m), hindbrain (h), and spinal cord. Undisrupted 12/101 staining
indicated that all embryos developed normal muscle (C, H, K, and N; top, lateral; bottom, dorsal). Scale bars: 1 mm. |