| |
FIG. 2. Activated MAPK expression becomes localized to the
nucleus of the animal cap during blastula stages and later is
observed to increase dorsally. (Aâ C) Animal views of cleared
embryos at stage 6 (A), stage 7 (B), and stage 8 (C) (magnified in
insets). Nuclear staining was observed to increase between stages 6
and 7 (see insets). By stage 9 (D) staining was observed in the animal
cap (ac) and marginal zone (mz), but not in the vegetal pole (vp). (E)
A sagittal section through a stage 9 embryo. Equal amounts of ac,
mz, and vp protein were analyzed by Western blot using the
activated MAPK antibody (F). Activated MAPK was detected in the
ac and mz, but not in the vp. An increase in activated MAPK
expression was observed prior to dorsal lip formation (G, magnified
in inset). (H) A sagittal section of the embryo pictured in G, dorsal
staining is magnified in the inset. Scale bar, 350 mm. |
