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Figure 2: mcl1 expression pattern suggests a role for this protein in axial organizer development. (A) RT-qPCR analysis of apoptotic cascade mRNA in Xenopus early neurula. Analysis performed on RNA extracted from dissected neural plates, and ventral ectoderms (N=15) of X. laevis embryos at stage (st.) 14 for pro-apoptotic factors – bak, bad, bid, bim, including caspases (casp) - casp3, casp7, and casp9 and anti-apoptotic factors – bcl2l10, mcl1, bcl-xl, and bcl2l2. Expression is normalized relative to ef1α. Relative normalized expression is indicated as means ± s.e. (B) mcl1 expression pattern. ISH using a probe for mcl1 from stage 11 to stage 20 embryos; (a) ventral view, dorsal up, (b-e) dorsal view anterior up, (f) anterior view dorsal up. (g) Transverse section of (c) in the posterior part of the embryo. The scale bar stands for 200μm. The white arrow indicates the border between the ectoderm and the neuroectoderm. The black arrow indicates the notochord. (C) bcl-xl expression pattern in X. laevis gastrula and neurula. ISH using a probe for the anti-apoptotic gene bcl-xl from stage 12 to stage 20. Embryos are shown dorsal side up. |
