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mmp9.1xenopus epidermis inner layer [+] 

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Experiment details for mmp9.1

Characterizing gene expression during lens formation in Xenopus laevis: evaluating the model for embryonic lens induction.

Characterizing gene expression during lens formation in Xenopus laevis: evaluating the model for embryonic lens induction.

Gene Clone Species Stages Anatomy
mmp9.1.S laevis NF stage 35 and 36 epidermis inner layer

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  Figure 3. Expression patterns of genes isolated from the subtracted cDNA library. RNA whole-mount in situ hybridization revealing the localization of six different mRNAs in Xenopus embryos. All whole-mount early larval stages are shown as right lateral views, with the anterior ends located to the right of the figure. All whole-mount embryos are shown as left dorsolateral views, with the anterior end directed toward the left side of the figure. A: Expression of B66 (Xmmp-9) at stage 35. Notice that expression is detected in several isolated mesenchymal cells, which lie just beneath the ectoderm. Most of these cells are located along the trunk and in the tail, and very few are seen in the head. B: Expression of B87 (Xenopus CRSP34) at stage 30. Note intense expression in the eye and lens (ey), as well as the otic vesicle (ot), pharyngeal arches (pa), spinal cord (sc), pronephros (pn), and axial musculature. The black arrowhead points to expression in the pineal gland. C: Expression of B99 (identity unknown) at stage 27. Note intense expression in the eye and lens, as well as one of the pharyngeal arches. Some weaker expression may also be seen in the otic vesicle. D: Expression of B105 (identity unknown) at stage 14. Expression is seen to the placodal ectoderm surrounding the anterior neural plate (np). The most intense expression is localized in the presumptive lens ectoderm (white arrowhead), whereas some expression is also detected in the area of the presumptive otic region (black arrowhead). Much weaker expression extends anteriorly into the region of the presumptive olfactory ectoderm. Thin lines of expression are also seen in the anterior neural folds (nf). E: Transverse section through the anterior neural plate showing expression in the presumptive lens ectoderm (ple) and cells in the neural folds. Relative plane of section is indicated by dotted white line shown in D. F: Expression of B105 (identity unknown) at stage 19. G: Expression of B105 at an even later stage of development (stage 34). Note intense expression in the eye and lens, as well as the otic vesicle. Asterisks mark the locations of segmental expression in the brain. Black arrowhead points to expression in the pineal gland. H: Cross-section through the eye and lens of a stage 30 larva showing expression in the lens (ln). rt, retina. I: Representative negative control showing no hybridization of sense D43 probe (D43s) to a stage 32 embryo. J: Expression of D43 at stage 15. Expression is seen in regions similar to those expressing B105. K: Expression of D43 at stage 32. Expression is detected in the head in structures including the eye, lens, and otic vesicle. Other expression is also seen in the spinal cord, the pronephros and pronephric duct, and in cells of the cloaca (cl). L: Expression of E7. Specimens shown in B and C have been cleared, whereas the others have not. Scale bars = 25 mu m in E, 30 mu m in H, 250 mu m in J (applies to D,F,J), 500 mu m in L (applies to A-C,G,I,K,L).