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Fig. 2. Embryonic expression patterns of Xenopus gelatinase B (Xmmp-9), revealed by digoxygenin-labeled RNA in situ hybridization. A: Lateral view of a stage 26 embryo showing the first detectable Xmmp-9 expression in scattered mesenchymal cells. B: Increased expression in a stage 37 embryo (black arrowhead points to expression associated with the developing liver diverticulum ). C: High magnification view of the tail of a stage 40 larva. D: Stage 42 larvae showing continued expression in the abdominal region and the tail. Faint staining in the head and in the olfactory epithelium is an artifact, which is sometimes seen in sense RNA controls. E: Stage 46 embryo showing decreased expression of Xmmp-9. A small number of pigmented xanthophores (xn), in the head, dorsal tail, and peritoneum normally appear in albino larvae at this stage, which are not expressing Xmmp-9. F: Negative control stage 38 embryo reveals no appreciable hybridization of sense RNA. All specimens are shown as lateral views with the anterior ends located to the left. cv, caudal veins; ey, eye; ms, mesenchyme cells. Scale bar =500 um in A,B and D and 250uminC. |
