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Fig. 3. Characterization of GFP-positive cells in limb stumps. (A) A longitudinal section of a limb blastema at 11 days stained with anti-GFP and anti-MSX1/2. The merge shows that MSX1/2-positive blastemal cells were GFP positive. (B) A longitudinal section of a limb blastema at 4 days stained with anti-GFP and anti-BrdU. The merge shows that cycling cells incorporating BrdU were GFP positive. (C, D) Numbers of BrdU- and phospho-Histone H3-positive cells in limb stumps. Note that reentry of mesodermal cells into the cell cycle became evident at 2 days after limb amputation. (E) Temporal transition of the percentage of GFP-positive cells in BrdU-positive cells, showing that most of the cycling cells were GFP positive. In all cases, points represent the average of at least four stumps. Scale bars: 200 μm. |
