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Figure 6. XMLC2 is a marker for terminal differentiation of
cardiac muscle in Xenopus embryos. The distribution of
XMLC2 RNA was examined by use of albino embryos and
digoxigenin-labeled probes. Staining was detected first in stage
28/29 tadpoles in the presumptive heart region and was subsequently
confined to the developing heart tube. A stage 32 embryo
is shown before (a) and after (b) clearing. In later stages,
staining was detected in both atrial and venticular chambers of
the beating tadpole heart. No staining was detected with a sense
control probe. |