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Figure 7. In situ-hybridization in the Xenopus lavies brain, using probes for selected genes. Xenopus laevis tadpoles sampled from Ex 6 hr (6 h exposure to predation), Ex 24 hr (24 h exposure to predations), Ex 10 days (10 days exposure to predation), and Ex 5 days-Out (5 days exposure to predation and then five days without) were used for in situ-hybridization using selected genes. (a) The red rectangle indicates the region of the brain used in this experiment. (b) Magnified image of the brain and the names of the brain regions. Tc, Dc, Ms, and Mt indicate the telencephalon, diencephalon, mesencephalon, and metencephalon, respectively. Antisense and sense probes were hybridized to the brain. In situ-hybridization results using anti-sense and sense probe for non-NMDA glutamate receptor (c), myosin light chain (d), hypoxia-inducible factor 1 alpha (e), erythropoietin receptor (f), and serpin 1 mRNA binding protein 1 (g). ① corresponds to the mammalian isocortex; ② indicates a ventral region of the posterior commissure, which may correspond to the subcommissural organ; ③ and ④ indicate the thalamus and hypothalamus in the diencephalon, respectively. |
