| Search Criteria |
| Gene/Clone | Species | Stage | Anatomy Item | Experimenter |
|---|---|---|---|---|
| nap1l1 | xenopus | blastomere [+] |
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Experiment details for nap1l1
Xenopus nucleosome assembly protein becomes tissue-restricted during development and can alter the expression of specific ge...Xenopus nucleosome assembly protein becomes tissue-restricted during development and can alter the expression of specific genes.
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| Fig. 5. xNAP1L is cytoplasmic prior to the MBT but enters nuclei subsequently. (A–F) Embryo sections were used for immunohistochemistry with either a pre-immune serum (A, C and E) or the anti-xNAP1L serum (B, D, F and G). (A–D) show sections from stage 6 embryo blastomeres and staining is visible throughout the cells except for the nuclei (upper panels) which are highlighted by DAPI staining (lower panels). (E–F) show sections through gastrula stage embryos, staining is strongest in the ectodermal and mesodermal cells but also seen in the endoderm. In all layers there is now nuclear staining (examples are arrowed in (F)) coincident with DAPI fluorescence (G). (H and I) Embryos were injected at the two cell stage with 100 pg of synthetic RNA encoding hemaglutanin-tagged xNAP1L and allowed to develop until stage 7(H) or 9(I). They were then fixed and the exogenous protein was detected by immunohistochemistry. In the stage 7 embryos staining is absent from nuclei (arrowed in (G)) but by stage 9 punctuate staining of nuclei is seen, although there is still substantial signal in the cytoplasm. |