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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
ubtf
|
|
laevis
|
NF stage 4 (8-cell)
|
animal pole
|
ubtf
|
|
laevis
|
NF stage 10
|
animal pole
|
ubtf
|
|
laevis
|
NF stage 18
to
NF stage 19
|
neuroectoderm
,
neural plate
|
ubtf
|
|
laevis
|
NF stage 26
|
otic vesicle
,
brain
,
presomitic mesoderm
,
pharyngeal arch
,
tail bud
,
[+]
|
ubtf
|
|
laevis
|
NF stage 33 and 34
|
otic vesicle
,
retina
,
brain
,
pharyngeal arch
,
eye
,
[+]
|
fbl
|
|
laevis
|
NF stage 4 (8-cell)
|
animal pole
|
fbl
|
|
laevis
|
NF stage 11
|
animal pole
|
fbl
|
|
laevis
|
NF stage 18
|
neural plate
|
fbl
|
|
laevis
|
NF stage 25
|
brain
,
presomitic mesoderm
,
pharyngeal arch
,
tail bud
,
eye
,
[+]
|
fbl
|
|
laevis
|
NF stage 32
|
otic vesicle
,
brain
,
presomitic mesoderm
,
pharyngeal arch
,
tail bud
,
[+]
|
ncl
|
|
laevis
|
NF stage 4 (8-cell)
|
animal pole
|
ncl
|
|
laevis
|
NF stage 10
|
animal pole
|
ncl
|
|
laevis
|
NF stage 18
|
epidermis
,
neural plate
|
ncl
|
|
laevis
|
NF stage 32
|
otic vesicle
,
brain
,
spinal cord
,
pharyngeal arch
,
tail bud
,
[+]
|
|
|
Fig 1. Spatiotemporal expression of fbl, ncl, and ubtf in Xenopus laevis during development.
A, fbl, ncl, and ubtf mRNAs were detected in X. laevis during development by whole mount in situ hybridization (WISH). The stages and, where relevant, the animal and vegetal poles are indicated. The schematics highlight the labeled structures at stage 18 (neurulation) and at stages 25–32 (organogenesis). NCCs, neural crest cells; H, hindbrain; M, midbrain; E, eyes; P, pronephros; BA, branchial arches. B, fbl expression was analyzed by RT-qPCR in animal cap explants injected either with noggin (to induce differentiation to neural tissue detected with sox3) or with noggin and wnt8 (to stimulate differentiation to neural crest tissue detected with slug). Non-induced (non-injected) cap explants naturally differentiate to epiderm detected with krt12. As a control, expression of the inspected genes was normalized to gapdh. fbl was most abundantly expressed in neural crest tissue. C, Spatial distribution of fbl mRNAs established by WISH in E14.5 mouse embryos. The schematics indicate the orientation of the section plane (dashed line). v, vibrissae; m, mesenchyma; c, primordium of mandibular cartilage; r, retina; sm, submandibular glands. |
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ncl (nucleolin) gene expression in Xenopus laevis embryo, via in situ hybridization, NF stage 25, lateral view, anterior right, dorsal up. |
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ncl (nucleolin) gene expression in Xenopus laevis embryo, via in situ hybridization, NF stage 32, lateral view, anterior right, dorsal up. |