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Fig. 6. Lateral line placodes are disrupted in XSmo- and mHIP-injected embryos. Embryos were injected with XSmo mRNA (1.5 ng, C, D; or 2 ng, A, B)
or mHIP mRNA (500 pg, E, F) into one blastomere of the two-cell stage, fixed at stage 28, and analyzed for NeuroD expression. High doses of injected XSmo
mRNA resulted in a strong disruption and reduction of the NeuroD-expressing placodal region (B, 44%, n 18) Convergence of ganglia from the
epibranchial placodes VII and IX (D, arrow) was observed in 33% (n 21) of embryos injected with low doses of XSmo mRNA. NeuroD expression was
also disrupted in 64% (n 25) of mHIP-injected embryos (F). epVII, facial epibranchial placode; epIX, glossopharyngeal epibranchial placode; epX1, first
vagal epibranchial placode; expX2/3, fused second and third vagal epibranchial placode; pAD, anterodorsal lateral line placode; pM, middle lateral line
placode; pP, posterior lateral line placode; is, injected side; nis, noninjected side. |
