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Fig. 2. XNIF encodes for a vegetally localized RNA in Xenopus oocytes. Whole-mount in-situ hybridization was carried out using albino oocytes of different stages. For a detailed analysis, sections of stained stage I and stage II oocytes were prepared. (A) In stage I oocytes, XNIF RNA is enriched in the mitochondrial cloud (examples marked by arrows). The cell nucleus is indicated by a dashed line in one oocyte. (B) In stage II oocytes, XNIF accumulates at the tip of the vegetal cortex (red arrow). In stage III-IV oocytes, XNIF RNA is associated with the vegetal cortex (black arrow). (C) Vegetal-cortical localization of XNIF in stage V/VI oocytes. (D) Section of whole-mount in-situ stained stage I oocyte. XNIF transcript is enriched in the mitochondrial cloud adjacent to the germinal vesicle. (E) Section of whole-mount in-situ stained stage II oocyte. XNIF is associated with reticular structures in a wedge-shaped region beneath the germinal vesicle. Scale bar:∼ 100 μm in (A,D,E) and 1 mm in (B,C). (F) XNIF expression in a stage 33 Xenopus albino embryo. Whole-mount in-situ hybridization staining with a XNIF specific antisense probe is shown. |
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neff1 (neuronal intermediate filament family member 1) gene expression in Xenopus laevis embryo, NF stage 32, assayed via in situ hybridization, lateral view, anterior left, dorsal up. |
