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Figure 4. Expression pattern of NM23-X3, -X4 and p27Xic1 in Xenopus retina. (A, B) Both NM23-X3 (A) and NM23-X4 (B) are expressed in the CMZ of Xenopus retina as shown by in situ hybridization on stage 41 cryosections. Red brackets indicate the expressed regions in the CMZ. (C) Sense-probe used as negative control. (D, E) NM23-X4 expression pattern in the retinal CMZ (D) overlaps with that of p27 (E), as underlined by dotted lines in blue and red, respectively. In situ hybridization was done at stage 39 sequential cryosections with signal visualized with BM purple and Fast Red, respectively. (E) Merged image; white dotted line marks the retinal epithelium boundaries. (G) A schematic diagram of expression of p27Xic1 and NM23-X4 or -X3 in embryonic Xenopus retina. |
