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Fig. 4. Electron microscopy and immunohistochemical observations of the heart chambers. (A,D) Electron micrographs of ventricular myocardium in the normal and ectopic hearts. Ectopic hearts showed similar structures to those observed in normal hearts. No ANP granules are apparent in either image. (B,E) Electron micrographs of atrial myocardium in the normal and ectopic hearts. Numerous ANP granules (arrows) are present in both. (C,F) Immunostaining of normal and ectopic hearts with anti-ANP antibody showed strong ANP expression in the atrium (a) and atrium-like region (a, but not in the ventricle (v) or ventricle-like region (v. Scale bars are 1 μm in (A,B,D and E); 200 μm in (C,F). |