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FIG. 3. Indirect immunofluorescence of MAb-A5 and MAb-B2 on cryostat sections of the mesencephalic region, located rostrally to the
sections shown in Fig. 1. (a) A coronal section stained with MAb-A5. The pretectum shows immunofluorescence (arrow). A small region
corresponding to the basal optic nucleus shows the faint fluorescence at the ventrolateral part (*) (X110). (b) The section next to (a) stained
with MAb-B2. The pretectum and the basal optic nucleus are negative. (c-e) Enhanced image showing localization of MAb-A5 binding in a
ventrolateral part of the mesencephalon processed by a color image analyzer: (c) integrated image (128 times) (X250), (d) specific object image
extracted as the highest area which corresponds to the basal optic nucleus, and (e) image in (d) superimposed on (c). |