| Search Criteria |
| Gene/Clone | Species | Stage | Anatomy Item | Experimenter |
|---|---|---|---|---|
| opa1 | xenopus | somite [+] |
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Experiment details for opa1
Hoffmeister M et al. (2017) AssayDevelopmental neurogenesis in mouse and Xenopus is impaired in the absence of Nosip.
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| Fig. 6. Nosip and Rbp1 act synergistically duringXenopusbrain development (A)rbp1-, opa1- and syne2-specific WMISH of Xenopus embryos at indicated stages; black arrowheads pointing to anterior neural plate at stage 13 and brain at stages 23 and 30, white arrowhead pointing to the eye, red arrowhead to pharyngeal arches (B)rbp1-specific WMISH of Xenopus embryos at stage 23 after unilateral injection of Nosip MO or Control MO as indicated; injected side at the right, arrowhead pointing to reduced rbp1 expression in the brain (C) Statistical evaluation of the data shown in B (D) Bright field images of Xenopus brains at stage 42, anterior to the top, after unilateral injection of Rbp1 MO or Control MO and GFP mRNA as indicated; scale bar 200 µm (E) Statistical evaluation of the data shown in D, N = number of independently evaluated brains (F) Synergy experiment. Bright field images of Xenopus brains at stage 42, after unilateral injection of either 10 ng Nosip MO or 30 ng Rbp1 MO alone or in combination. 0.5 ng GFP mRNA was co-injected as lineage tracer; scale bar 200 µm (G) Statistical evaluation of the data shown in F, N = number of independently evaluated brains. | |||||||||||
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| opa1 (optic atrophy 1 (autosomal dominant)) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 30, lateral view, anterior right, dorsal up. |