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FIG. 3. Whole-mount immunocytochemistry of embryos. (A) Skin dissected from stage 25 whole-mount embryo labeled with antigen affinity purified L13 pAb (see Materials and Methods). (B) Skin dissected from control stage 25 whole-mount embryos labeled with antigen affinity-purified preimmune sera. (C) Stage 41 whole-mount embryo stained with xlcaax-1 pAb. The arrows indicate the specific staining of the pronephric
tubule. (D) Control stage 41 whole-mount embryo. Arrows indicate pronephric area with no specific staining. (E) Higher magnification of stage 41 pronephros. al, ascending limb; act, anterior convoluted tubule; dl, descending limb, w, wolffian duct. (Parts of pronephros from Tytler, 1933). Magn. of A and B is 1000~. Magn. of C and D is 25X. Magn. of E is 60X. |
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FIG. 4. Accumulation of xlcaax-1 protein in differentiated skin, pronephros and mesonephros. Immunocytochemistry of embryonic tissue sections stained with the L13 pAb. (A) Stage 15/16 embryos, arrows indicate undifferentiated outer ectoderm. (B) Stage 23/24 embryo, arrows indicate differentiated epidermis with basolateral xlcaax-1 staining. (C) Stage 29136 embryo, arrows indicate pronephric tubules in initial stages of differentiation. (D) Stage 35 embryo, arrows indicate differentiated pronephric tubules. (E) Stage 47/48 embryo, arrows indicate undifferentiated mesonephric tissue, open arrows indicate the pronephric ducts. (F) Stage 49 embryo showing specific xlcaax-1 staining in the tubules, open arrow indicates the pronephric duct, curved arrow indicates the distal tubule, arrow indicates the proximal tubule, s, skin. Magn. is 500X. |
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FIG. 8. Electron microscopy of distal tubule. (A) Preimmune, Protein A purified IgC control. (B) L13 pAb specific binding over the basal
infoldings of the plasma membranes of distal tubules. (C) High magnification showing localization to the cytoplasmic aspect of the basal
plasma membrane infoldings. Magn. of A and B is 14,909~. Magn. of C is 49,799x. |
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FIG. 7. Xlcaax-1 staining in the distal tubule of the kidney. (A) Section of adult kidney stained with L13 pAb, box indicates region shown in C.
(B) Control section of adult kidney. (C) High magn. of boxed area in A, arrows indicate the basolateral membrane, box indicates region shown in
Fig. 8. d, distal tubule; p, proximal tubule; g, glomerulus; n, nucleus; lu, lumen. Magn. of A and B is 160~. Magn. of C is 1000X. |