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Figure 2. Decreased expression of hspa9 following in vivo reduction of RA signaling. A: Analysis of the GFP tracer distribution on one side of cyp26-injected embryos at neurula stage. B–I: pax8 ISH of cyp26-injected embryos at 0.15 ng (B,D,F,H) or 0.50 ng (C,E,G,I) dose was performed at st14. The otic territory (ot) and kidney field (kf) are specifically labeled by pax8 (D–G) and show a strongly reduced (F) or absent (G) pax8 positive renal field as already noticed on dorsal views (red arrows in B and C). Similar observation is made at st25 on the side of cyp26-injected embryos (H, I). J–Q: Expression of hspa9, pax8 and pax2 upon reduced RA signaling. At st24, hspa9 and pax8 are significantly reduced on the cyp26-injected side in the pronephros area (arrows, K,M). Hspa9 expression is reduced in the most anterior somites areas (white bracket) and branchial arch (*) as well (K). Similar results are obtained when compared with pax2 at late tadpole stage (O,Q). The nephric duct elongation was unaffected at the 0.15 ng dose of cyp26 injection (arrows in O,Q).Download figure to PowerPoint |
