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Fig. 7. Localization of XDCoH in the Xenopus embryo. Whole-mount staining of different development stages was performed using an affinity-purified polyclonal XDCoH specific antiserum as the rst antibody and an enzyme linked secondary antibody. (A) The upper 4-cell stage embryo is a control for antibody specifity and was incubated with the XDCoH-specific antiserum that was depleted using recombinant XDCoH protein. (B,C) A lateral and animal view of a 48-cell stage embryo (stage 6.5),
respectively. (D,E) Blastulae at stage 8 and 9, respectively, and (F) a gastrula (stage 11). (G,H) XDCoH-specific stained organs are the pronephros (p), eye vesicle (e), gut and liver (g, l) and the pigmented epithelium (pe). |
