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Fig 2. pkdcc1 and pkdcc2 expression patterns during X. laevis embryonic development.
(A-H) Expression pattern of pkdcc1 during early development. (I) Temporal expression pattern of Xenopus pkdcc1 and pkdcc2 analysed by quantitative PCR. GAPDH was used as reference gene. (J-O’) Expression pattern of pkdcc2 during Xenopus development. By early gastrula stages, pkdcc1 transcripts can be detected in the (A) dorsal blastopore lip and (B) ADE. (C) At stage 12, pkdcc1 is present in the involuting mesoderm. (D) During neurula stages pkdcc1 is expressed in neural folds and eye fields. (E-F’) pkdcc1 expression at tailbud stages is restricted to the lateral plate mesoderm, foregut, eye and isthmus. (G) Double whole mount in situ hybridization for pkdcc1 (blue) and otx2 (red) shows that pkdcc1 expression in the brain is restricted to the mid-hindbrain boundary. (H) Double whole mount in situ hybridization for pkdcc1 (blue) and cardiac troponin (red) showed that pkdcc1 expression is absent on the heart. (J) Zygotic pkdcc2 is detectable at early gastrula stages in the anterior dorsal endoderm. (K) At late-gastrula stages pkdcc2 mRNA is expressed in the involuting mesoderm. (L) During early neurula stages, pkdcc2 transcripts can be detected in the neural folds (dorsal view, anterior down). (M) At stage 17, pkdcc2 is co-expressed with pkdcc1 in the prospective eye field and neural folds. (N-O’) In tailbud stages (27 and 31; lateral view, anterior to the right and dorsal up), pkdcc2 is expressed in the neural tube roof, otic vesicle, notochord, eye, lateral plate mesoderm and head mesenchyme. D, dorsal; V, ventral; A, anterior; P, posterior. |
