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Figure 2. In situ hybridization screen reveals new expression patterns. A: Embryos at gastrula (10.5), neurula (15), and tailbud (30) stage are stained with antisense (left) and sense (right) probes for EST-11 showing expression in the mesoderm, neural tube, neural crest, and pronephros. B: Embryos at neurula and tailbud stage are stained with antisense and sense probes for EST-10 demonstrating expression in cement gland, neural tube nasal placode, otic placod, and forebrain. C: Embryos at gastrula and tailbud stage are stained for antisense and sense probes for EST-14 demonstrating expression in mesoderm and in a single posterior somite. D: Embryos at neurula and tailbud stage are stained with antisense and sense probes for EST-8, indicating expression within forebrain and heart. E: Embryos at neurula and tailbud stage are stained with antisense and sense probes for EST-34, showing expression in the placodes. F: Embryos at tailbud stage are stained with antisense and sense probes for EST-28 showing expression in nasal placodes and hindbrain. G: Embryos at neurula stage are stained with antisense and sense probes for EST-19, indicating expression within the neural tube. H: Embryos at tailbud stage are stained with antisense and sense probes for EST-9, demonstrating expression within pronephros and neural crest. |
