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Fie. 8. Whole mount in situ hybridisation to embryos and larvae using an antisense XLPOU-60 probe. (a) Transverse section of a blastula
(stage 8) embryo. A blue colour indicates positive staining. The transcript is perinuclear and is localised to the animal cap and marginal zone
cells of the embryo. No staining is seen surrounding nuclei in the vegetal region (black arrowheads). Some superficial cells of the animal cap and
marginal zone appear to lack the transcr ipt (white arrows). (b) Dissected region of a stage 44 larvae. No blue stain is seen on the surface of the
dorsal body wall, where the primordial germ cells are located at this stage (arrowhead). Some blue stain is seen in the tail fin, but this is
nonspecific; it was also present in larvae hybridised to a sense probe. (c) Drawing of a stage 44 tadpole to explain the dissection in (b). The
dashed region was removed, leaving the tail. and the exposed dorsal body wall, with attached primordial germ cells (solid line). The dotted box
indi~ates the region enlarged in (d) and (c). (d) Stage 44 tadpole as in (b), showing the primordial germ cells (arrowheads). No blue colour is
detectable in these cells. (e) Drawing of (d) shows the position of the primordial germ cells on the dorsal body wall (arrowheads). |
