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Fig. 4. Spatial expression profile of X-CypA during development. Whole-mount in situ hybridisation with an X-CypA DIG-labelled antisense RNA probe was performed on embryos from stages 9–39 as indicated in the figure. Expression was first detected in the nervous system from stage 15 (B), with apparent highest expression in the anterior forebrain. This distribution persists throughout neurulation and early tailbud stages (C–E). At stage 26, X-CypA expression appears in the sensory organs including the eyes and otic vesicles and is maintained throughout their organogenesis (F–L). From stage 28, the gene is expressed in the pronephric kidney (H, I and M). At stage 39, expression can be clearly detected in the developing lateral line system (F). Panels A–C show embryos cleared in Murrays, panels D, K–M are uncleared embryos and panels F–J uncleared embryos after treatment in 70% ethanol. The sense probe showed no staining pattern (data not shown). ans, anterior nervous system; pn, pronephros; pd, pronephric duct; cg, cement gland; ey, eyes; ov, otic vesicle; ba, branchial arches; ll, lateral line. |
