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Fig. 2. Temporal and spatial distribution of xPRMT1b RNA expression during embryogenesis. (A) RT-PCR analysis of RNA extracted from oocyte (ovo) and embryos at the indicated stages. (B) Whole-mount ISH analysis of the tissue distribution of xPRMT1b transcripts (1). Stage 10.5, vegetal view (2). Corresponding sagittal section (animal side up, dorsal side to the left) (3). Stage 20, dorsal view (4). Corresponding cross section of the trunk region (dorsal side up) (5). Tailbud embryo, dorsal view (6). Corresponding anterior transverse section. a, anterior; ba, branchial arches; cg, cement gland; d, dorsal; hb, hindbrain; il, inner layer; mb, midbrain; nc, notochord; nt, neural tube; ol, outer layer; ov, optic vesicle; p, posterior; *, dorsal blastopore lip. (C) LTC antagonist represses the expression of xPRMT1b. Embryos were treated from stage 8 to 10 with 300 μM nifedipine. xPRMT1b expression was revealed by ISH (dorsal views, blastopore to the top) at stage 10.5 (Left) and stage 12.5 (Right). |
