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Fig. 3. Spatial expression profiles of polypyrimidine tract binding protein (PTB) genes during early Xenopus development. Whole-mount in situ
hybridization was performed to study the spatial expression of ptbp1, ptbp2 and ptbp3 at different developmental stages. (A). ptbp1 (a) lateral view
of an unfertilized egg (UFE), (a UFE bisected through the animal-vegetal axis, (b) lateral view of a blastula, (b blastula bisected through the animalvegetal
axis, (c) lateral view of a gastrula, (c vegetal view of panel c, (c) gastrula bisected through the animal-vegetal axis, (c higher magnification
view of the boxed area in panel c, (d-d dorsal views of neurula stages with two different detection times, (d lateral view of panel d (e) lateral view
of an early tailbud embryo at stage 24. sox17` (f) vegetal view of a gastrula, (f gastrula bisected through the animal-vegetal axis. (B) ptbp2 (a) lateral
view of an UFE, (b) lateral view of a blastula, (c) lateral view of a gastrula, (d) dorsal view of neurula stage, (d lateral view of panel d, (e) lateral view
of an early tailbud embryo at stage 24, (e dorsal view of panel e. (C) ptbp3 (a) lateral view of an UFE, (b) lateral view of a blastula, (c) lateral view of a
gastrula, (d) ventral view of an early tailbud embryo at stage 20, (d lateral view of panel d, (e) lateral view of an early tailbud embryo at stage 24, (e
ventral view of panel e. Abbreviations used are: ba, branchial arches; br, brain; ec, ectoderm; en, endoderm; ey, eye; mes, mesoderm; nc, neural crests;
nt, notochord; op, otic placode; pn, pronephros, vbi, ventral body island; sc, spinal cord. |
