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Fig. 2.
Normal floor plate induction and Hedgehog signaling in ATP4a-deficient embryos. (A–E) Normal floor plate formation in atp4a morphants. WMISH for foxj1 (A–C) and shh (D, E) revealed attenuated foxj1 expression but unaffected floor plate formation, as judged by apically constricted cells (histological vibratome sections in A–C`; planes indicated in A–C) and shh expression in atp4a morphants. (F–K) Unaffected Hedgehog signaling in atp4a morphants with (H) or without (G) co-injection of ß-catenin (β-cat.) DNA, as judged by WMISH for ptch1, a direct Hedgehog signaling target, to monitor activity of Hedgehog signaling, as compared to uninjected controls (uninj.). ptch1 expression was decreased in cyclopamine-treated embryos (J, K), as compared to ethanol (1%) controls (I), but independent of β-catenin DNA injection (K, K`). a, anterior; l, left; p, posterior, right; st., stage. |
