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Fig. 7. Xbf2 participates in patterning of the forebrain. All the embryos are shown from anterior side. The weaker expression of Xbf2 in neural crest cells is not visible in A and B due to low staining development. (A) Double in situ hybridization of an stage 17 embryo. Blue (arrow) corresponds to Xbf2 expression and purple (arrowhead) denotes Xbf1 expression. (B) Xbf2 expression in an stage 17 embryo injected in one blastomere of a 2-cell stage embryo with 0.5 ng of Xbf1 mRNA and 0.3 ng of lacZ mRNA. Notice the complete inhibition of Xbf2 expression at the injected side (58%, n = 12). (C) Embryo injected in one blastomere of a 2-cell stage embryo with 0.25 ng of Xbf2 mRNA and 0.3 ng lacZ mRNA and cultured until stage 17. Xbf1 expression is suppressed in the injected side (72%, n = 22). (D) Similar embryo as in C, but expression of Xcpl1 was analyzed. Notice the inhibition of Xcpl1 in the injected side (53%,n = 15). |
