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Fig. 3. Xpygo-2 / are differentially expressed within the brain. Whole-mount in situ hybridization analysis of staged embryos using probes complementary
to both transcripts (A–H) or specific to Xpygo-2 mRNA (I–O), or sense probes, where indicated (B, D, F, H, J, M, O). Specific hybridization is indicated
by purple staining reaction. All embryos are shown with anterior to the left. Xpygo-2 mRNA is found early (St 13) in the anterior neural plate (A, dorsal view).
By stage 20 and at least until stage 25, messages localized within the anterior neural tube, including prospective brain and eyes (C and E, lateral views) and
in the tadpole, in the mid–hindbrain, forebrain, and eyes (G, lateral view). Xpygo-2 transcripts are present throughout blastulae (I, animal pole view), but
are restricted to the retinal field at stage 20 (K, dorsal view) and in derivatives of this region (forebrain and eyes) at stage 25 (L, lateral view) and stage 30
(N, lateral view). |
