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Fig. 1 (Left). XRASGRP expression precedes Ami expression. (A) XRASGRP2 transcripts localized in the anterior cardinal vein (ACV), aortic arch (AA), intersomitic veins (ISV), posterior cardinal veins (PCV), and vascular vitelline network (VVN) at stage 30. (B) XRASGRP2 expression is detected in the PCV and VVN at stage 35. Lines indicate the positions of the sections shown in (G-J). (C) At stage 40, XRASGRP2 expression is restricted to the PCV and VVN. (D) The expression of Ami is weakly detected in the ACV, AA, and VVN at stage 30. (E) Ami expression is evident in the ACV, AA, ISV, PCV, and VVN at stage 35. Lines indicate the positions of the sections shown in (K-N). (F) Ami expression is detected continuously in the PCV, ISV, and VVN until stage 40. (G-J) Histologic section of the embryo shown in (B). (K-N) Histologic section of the embryo shown in (E). Both XRASGRP2 and Ami are expressed in the endothelial cells (PCV and VVN). |
