|
Display additional annotations [+]
|
| |
Fig. 2. Expression pattern of Xenopus REEP4. (A) RT-PCR based expression profile of X. laevis REEP4 from stage 1 to tailbud stage 35. Values have been normalized to those of ornithine decarboxylase (ODC). (B-F) Whole-mount in situ hybridisation analysis of X. laevis REEP4 expression. (G-H) Transverse sections of X. laevis embryos at stage 30. Note expression of REEP4 in neural tube, otic vesicles and somites. (I,J) Expression of X. tropicalis REEP4 at tailbud stage 30 and tadpole stage 42. Note strong expression in somites in (I) and in the nervous system in (J). (K,L) REEP4 localises to plasma and nuclear membranes. Embryos at the one-cell stage were left uninjected (J) or received injections of 2 ng RNA encoding C-terminally GFP-tagged REEP4 (I). They were examined at stage 15. Note presence of tagged REEP4 in nuclear and plasma membranes. |
